Recombinant:Article Title: Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking
Article Snippet: DOI: https://doi.org/10.7554/eLife.82218 22 of 36 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- HA (mouse monoclonal) EMD Millipore 05- 904 (1 μg/ml dilution) Antibody Anti- GFP (mouse monoclonal) YenZym https://www.yenzym. com/default.html (0.1 mg/ml) Antibody Alexa 488- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A11036 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11036 (1:10,000 dilution) Antibody Alexa 488- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A32723 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11031 (1:10,000 dilution) Recombinant DNA reagent 6His- PreScission- DCX- EGFPStrepII (plasmid) Addgene #83918 Recombinant DNA reagent kif5b(1- 560)- EGFP- 6His (plasmid) Addgene #15219 Recombinant DNA reagent Sfp- 6His (plasmid) Addgene #75015 Recombinant DNA reagent Construct pGEX- 4T- 1 encoding JIP3 (plasmid) Gift from Dr. Valeria Cavalli; Sun et al., 2011 Recombinant DNA reagent Construct pCDNA3 encoding JIP3 (plasmid) Gift from Dr. Roger Davis; Kelkar et al., 2000 Recombinant DNA reagent pSNAP- tag(T7)2 (plasmid) NEB NEB #N9181S Recombinant DNA reagent pFastBac plasmid with codonoptimized full- length human dynein (plasmid) A gift from the Carter lab; Schlager et al., 2014 Recombinant DNA reagent The pFastBac plasmid encoding tail- truncated human dynein (amino acids 1320–4646) (plasmid) Gift from the ReckPeterson Lab; Htet et al., 2020 Recombinant DNA reagent Construct expressing IC- 1B (plasmid) Gift from Dr. Kevin Pfister; Ha et al., 2008 Recombinant DNA reagent Construct expression TrkB- RFP (plasmid) Gift from Dr. Xiaowei Zhuang Recombinant DNA reagent Plasmid pBA (plasmid) Gift from Dr. Gary Banker; Jacobson et al., 2006 Recombinant DNA reagent The pBA plasmid encoding HA- tagged N- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged C- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged DCX mutant T203R (plasmid) This paper Available upon request ‘Materials and methods’ Continued Continued on next page Fu et al. eLife 2022;11:e82218.
Plasmid Preparation:Article Title: Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking
Article Snippet: DOI: https://doi.org/10.7554/eLife.82218 22 of 36 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- HA (mouse monoclonal) EMD Millipore 05- 904 (1 μg/ml dilution) Antibody Anti- GFP (mouse monoclonal) YenZym https://www.yenzym. com/default.html (0.1 mg/ml) Antibody Alexa 488- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A11036 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11036 (1:10,000 dilution) Antibody Alexa 488- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A32723 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11031 (1:10,000 dilution) Recombinant DNA reagent 6His- PreScission- DCX- EGFPStrepII (plasmid) Addgene #83918 Recombinant DNA reagent kif5b(1- 560)- EGFP- 6His (plasmid) Addgene #15219 Recombinant DNA reagent Sfp- 6His (plasmid) Addgene #75015 Recombinant DNA reagent Construct pGEX- 4T- 1 encoding JIP3 (plasmid) Gift from Dr. Valeria Cavalli; Sun et al., 2011 Recombinant DNA reagent Construct pCDNA3 encoding JIP3 (plasmid) Gift from Dr. Roger Davis; Kelkar et al., 2000 Recombinant DNA reagent pSNAP- tag(T7)2 (plasmid) NEB NEB #N9181S Recombinant DNA reagent pFastBac plasmid with codonoptimized full- length human dynein (plasmid) A gift from the Carter lab; Schlager et al., 2014 Recombinant DNA reagent The pFastBac plasmid encoding tail- truncated human dynein (amino acids 1320–4646) (plasmid) Gift from the ReckPeterson Lab; Htet et al., 2020 Recombinant DNA reagent Construct expressing IC- 1B (plasmid) Gift from Dr. Kevin Pfister; Ha et al., 2008 Recombinant DNA reagent Construct expression TrkB- RFP (plasmid) Gift from Dr. Xiaowei Zhuang Recombinant DNA reagent Plasmid pBA (plasmid) Gift from Dr. Gary Banker; Jacobson et al., 2006 Recombinant DNA reagent The pBA plasmid encoding HA- tagged N- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged C- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged DCX mutant T203R (plasmid) This paper Available upon request ‘Materials and methods’ Continued Continued on next page Fu et al. eLife 2022;11:e82218.
Construct:Article Title: Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking
Article Snippet: DOI: https://doi.org/10.7554/eLife.82218 22 of 36 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- HA (mouse monoclonal) EMD Millipore 05- 904 (1 μg/ml dilution) Antibody Anti- GFP (mouse monoclonal) YenZym https://www.yenzym. com/default.html (0.1 mg/ml) Antibody Alexa 488- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A11036 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11036 (1:10,000 dilution) Antibody Alexa 488- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A32723 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11031 (1:10,000 dilution) Recombinant DNA reagent 6His- PreScission- DCX- EGFPStrepII (plasmid) Addgene #83918 Recombinant DNA reagent kif5b(1- 560)- EGFP- 6His (plasmid) Addgene #15219 Recombinant DNA reagent Sfp- 6His (plasmid) Addgene #75015 Recombinant DNA reagent Construct pGEX- 4T- 1 encoding JIP3 (plasmid) Gift from Dr. Valeria Cavalli; Sun et al., 2011 Recombinant DNA reagent Construct pCDNA3 encoding JIP3 (plasmid) Gift from Dr. Roger Davis; Kelkar et al., 2000 Recombinant DNA reagent pSNAP- tag(T7)2 (plasmid) NEB NEB #N9181S Recombinant DNA reagent pFastBac plasmid with codonoptimized full- length human dynein (plasmid) A gift from the Carter lab; Schlager et al., 2014 Recombinant DNA reagent The pFastBac plasmid encoding tail- truncated human dynein (amino acids 1320–4646) (plasmid) Gift from the ReckPeterson Lab; Htet et al., 2020 Recombinant DNA reagent Construct expressing IC- 1B (plasmid) Gift from Dr. Kevin Pfister; Ha et al., 2008 Recombinant DNA reagent Construct expression TrkB- RFP (plasmid) Gift from Dr. Xiaowei Zhuang Recombinant DNA reagent Plasmid pBA (plasmid) Gift from Dr. Gary Banker; Jacobson et al., 2006 Recombinant DNA reagent The pBA plasmid encoding HA- tagged N- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged C- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged DCX mutant T203R (plasmid) This paper Available upon request ‘Materials and methods’ Continued Continued on next page Fu et al. eLife 2022;11:e82218.
Expressing:Article Title: Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking
Article Snippet: DOI: https://doi.org/10.7554/eLife.82218 22 of 36 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- HA (mouse monoclonal) EMD Millipore 05- 904 (1 μg/ml dilution) Antibody Anti- GFP (mouse monoclonal) YenZym https://www.yenzym. com/default.html (0.1 mg/ml) Antibody Alexa 488- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A11036 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11036 (1:10,000 dilution) Antibody Alexa 488- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A32723 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11031 (1:10,000 dilution) Recombinant DNA reagent 6His- PreScission- DCX- EGFPStrepII (plasmid) Addgene #83918 Recombinant DNA reagent kif5b(1- 560)- EGFP- 6His (plasmid) Addgene #15219 Recombinant DNA reagent Sfp- 6His (plasmid) Addgene #75015 Recombinant DNA reagent Construct pGEX- 4T- 1 encoding JIP3 (plasmid) Gift from Dr. Valeria Cavalli; Sun et al., 2011 Recombinant DNA reagent Construct pCDNA3 encoding JIP3 (plasmid) Gift from Dr. Roger Davis; Kelkar et al., 2000 Recombinant DNA reagent pSNAP- tag(T7)2 (plasmid) NEB NEB #N9181S Recombinant DNA reagent pFastBac plasmid with codonoptimized full- length human dynein (plasmid) A gift from the Carter lab; Schlager et al., 2014 Recombinant DNA reagent The pFastBac plasmid encoding tail- truncated human dynein (amino acids 1320–4646) (plasmid) Gift from the ReckPeterson Lab; Htet et al., 2020 Recombinant DNA reagent Construct expressing IC- 1B (plasmid) Gift from Dr. Kevin Pfister; Ha et al., 2008 Recombinant DNA reagent Construct expression TrkB- RFP (plasmid) Gift from Dr. Xiaowei Zhuang Recombinant DNA reagent Plasmid pBA (plasmid) Gift from Dr. Gary Banker; Jacobson et al., 2006 Recombinant DNA reagent The pBA plasmid encoding HA- tagged N- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged C- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged DCX mutant T203R (plasmid) This paper Available upon request ‘Materials and methods’ Continued Continued on next page Fu et al. eLife 2022;11:e82218.
Mutagenesis:Article Title: Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking
Article Snippet: DOI: https://doi.org/10.7554/eLife.82218 22 of 36 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- HA (mouse monoclonal) EMD Millipore 05- 904 (1 μg/ml dilution) Antibody Anti- GFP (mouse monoclonal) YenZym https://www.yenzym. com/default.html (0.1 mg/ml) Antibody Alexa 488- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A11036 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- rabbit IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11036 (1:10,000 dilution) Antibody Alexa 488- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A32723 (1:10,000 dilution) Antibody Alexa 568- conjugated goat anti- mouse IgG (H+L) (goat polyclonal) Thermo Fisher Scientific A- 11031 (1:10,000 dilution) Recombinant DNA reagent 6His- PreScission- DCX- EGFPStrepII (plasmid) Addgene #83918 Recombinant DNA reagent kif5b(1- 560)- EGFP- 6His (plasmid) Addgene #15219 Recombinant DNA reagent Sfp- 6His (plasmid) Addgene #75015 Recombinant DNA reagent Construct pGEX- 4T- 1 encoding JIP3 (plasmid) Gift from Dr. Valeria Cavalli; Sun et al., 2011 Recombinant DNA reagent Construct pCDNA3 encoding JIP3 (plasmid) Gift from Dr. Roger Davis; Kelkar et al., 2000 Recombinant DNA reagent pSNAP- tag(T7)2 (plasmid) NEB NEB #N9181S Recombinant DNA reagent pFastBac plasmid with codonoptimized full- length human dynein (plasmid) A gift from the Carter lab; Schlager et al., 2014 Recombinant DNA reagent The pFastBac plasmid encoding tail- truncated human dynein (amino acids 1320–4646) (plasmid) Gift from the ReckPeterson Lab; Htet et al., 2020 Recombinant DNA reagent Construct expressing IC- 1B (plasmid) Gift from Dr. Kevin Pfister; Ha et al., 2008 Recombinant DNA reagent Construct expression TrkB- RFP (plasmid) Gift from Dr. Xiaowei Zhuang Recombinant DNA reagent Plasmid pBA (plasmid) Gift from Dr. Gary Banker; Jacobson et al., 2006 Recombinant DNA reagent The pBA plasmid encoding HA- tagged N- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged C- DCX mutant (plasmid) This paper Available upon request ‘Materials and methods’ Recombinant DNA reagent The pBA plasmid encoding HA- tagged DCX mutant T203R (plasmid) This paper Available upon request ‘Materials and methods’ Continued Continued on next page Fu et al. eLife 2022;11:e82218.
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